SLICSel is a program for designing specific oligonucleotide probes for microbial detection and identification. To obtain maximal specificity of designed oligonucleotides, SLICSel uses the Nearest-Neighbor thermodynamics-based approach for probe design.
Here is a sample target file and sample non-target file.
For further information please contact: and please check the HELP page.
There have been 22488 submissions to this web site since June 2007.
Probe melting temperature interval (min - max):
Annealing temperature for calculations will be:
Minimal binding energy (ΔG) difference between target and non-target sequences:
Maximal binding energy (ΔG) difference between target sequences:
Probe length, default:
Number of mismatches required with non-target sequences, default:
Concentration of monovalent cations ([Na]), default:
Concentration of nucleic acids ([target molecules]), default:
Check the formation of probe secondary structures (mfold)?
University of Tartu, Department of Bioinformatics 2022