1. Development of biotechnology-based food allergen surveillance methods
Project manager: Mart Ustav, Institute of Technology
Partners: Tartu University Hospital, Icosagen Cell Factory OÜ, The Association of Estonian Food Industry, Naxo OÜ
2. Estonia’s Integration to the European Bioinformatics Infrastructure (ELIXIR)
Duration: 01.11.2012 – 31.08.2015
Project manager: Jaak Vilo, UT Institute of Computer Science
Partners: Tallinn University of Technology, Estonian Biocenter, National Institute of Chemical and Biological Physics, University of Tallinn
Abstract: 1. Maintenance, upgrade and integration of bioinformatics tools and databases produced by Estonian research community (g:Profiler for gene list characterisations, MEM for large scale gene expression data analysis and queries, ESCDb – Embryonic stem cell database, etc.)
2. Interfacing with BBMRI through partnering with Estonian Biobank (Estonian node has expertise in data management solutions for health data, and Estonian medical records) and e-health data
3. Developing, offering, and intermediating training on bioinformatics and infrastructure that goes beyond standard university course offering
4. Developing Estonian node to match the future needs of bioinformatics data analysis, e.g. in next generation sequencing and respective service offering – building links to high-performance computing facilities in Estonia and through ELIXIR, porting bioinformatics solution on the cloud and more cost effective hardware (FPGA).
3. CTG – Centre of Translational Genomics
Duration: 2011 – 2015
Program: Development Fund of the University of Tartu
Abstract: The activities of the project are focused on discovering the genetic, epigenetic, transcriptomic, proteomic and metabolomic components lying behind common and complex diseases. The project will facilitate a systemic approach to the translation of genetic information from the DNA variations to the metabolic variations including also the RNA, epigenetic and proteomic levels.
4. Computational studies of genome sequences
Program: SF 2009
Abstract: Current grant application is focused to analysis and comparison of genome sequences of various organisms, mainly human genome. Throughout the project mainly bioinformatics and statistical methods are used for research. Experimental data is obtained from public databases or from collaborating partners. Firstly, we plan to analyze novel types of variations in human genome – gene copy number variations, inversions and traces of integrated viral genomes. Secondly, we plan to participate in larger projects which search for correlations between genes and human traits or diseases. Our part would be creation and use of novel statistical and bioinformatics methods for analyzing genomic data. Third part of the project will be devoted to comparison of non-human genome sequences. Our aim in this part of the project is discovery of novel universally conserved functional DNA elements. Our first target is to identify conserved upstream Open Reading Frames (uORFs) in bacterial genomes.
5. Centre of Excellence in Genomics
Project: TK10, 3.2.0101.08-0011
Financer: Archimedes Foundation
Abstract: The Centre of Excellence in Genomics is based on three workgroups from University of Tartu, and Estonian Biocentre, with its focus on basic and applied research of human as well as other genomes. The main objective of this consortium is to achieve, through dedicated collaboration, an added value, to be materialized and manifested in high-level scientific publications, enhanced international collaboration, creation of intellectual property and promotion of entrepreneurship in biotechnology. A particular goal will lie in applications in healthcare and in enhancement of the value of Estonian Biobank as a valuable national asset. It is an interdisciplinary research consortium with a scope extending from creating tools to analyse genomic, proteomic and metabolomic information and search for “disease genes”, to the understanding of the origin of genetic structure variation in humans.
6. Estonian Scientific Computing Infrastructure
Project: The High Performance Computing Center
Abstract: The High Performance Computing Center is a consortium of UT and it’s purpose is to build and develop the necessary infrastructure for scientific computing. It also manages and coordinates the use of said computing equipment. This computing resource is open for use to any research groups inside the university. Users from other Estonian science- and research institutions are also welcome.
7. Designing primers and hybridization samples suitable for complex diagnostics
Project: LLOMR 10049
Financer: Quattromed HTl Laborid OÜ
Abstract: HPV detection panel. Amplifing scheme for 42 different human papillomavirus (HPV) strain with the minimum number of universal primers, and 2-5 samples of strain-specific Luminex samples for each starin. Preferably, use regions E6/E7, if necessary, add LNA or other modified nucleotides.
Cervical pathogens primer panel. Designing PCR primers and Luminex hybridization samples for STD causing bacteria and viral pathogens. In total we will design primer pairs for about 14 different kinds / types of pathogens. For each pathogen we design 2-5 primers and 2-5 Luminex samples for testing purposes. If necessary, other modified nucleotides or LNA will be added to primers.
Panel of diarrhea pathogens. Designing PCR primers and Luminex hybridization samples for diarrhea causing bacteria and viral pathogens. In total we will design primer pairs for about 20 different kinds / types of pathogens. For each pathogen we design 2-5 primers and 2-5 Luminex samples for testing purposes. If necessary, other modified nucleotides or LNA will be added to primers.
Offering bioinformatic support services for the above-mentioned activities and for the activities related to EAS project” Enhancing complex diagnostics based on biotechnological platforms for healthcare system.
8. Design of PCR primers and Luminex hybridization probes
Project: LLOMR 10018
Financer: Quattromed HTl Laborid OÜ
Abstract: In the first step, species-specific PCR primers consisting only DNA nucleotides are designed for 6 bacterial pathogens. For each species 2-5 PCR primer pairs are designed. The species under investigation areChlamydia trachomatis, Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasmagenitalium and Trichomonas vaginalis. Luminex hybridization probes are designedfor PCR products that are amplified by previously designed PCR primers. In thisstep, Luminex probes consist only DNA nucleotdes also.For each PCR product 2-5 hybridization probes are designed. Thereafter the sequences are tested in the lab.
Oligos, designed in the first step, are redesigned. Into the PCR primer sequences 2-3 LNA nucleotides are added, so that PCR primers preserve similar melting temperature and temperature appropriatefor PCR. In the case of Luminex probes, different sites in probes are considered for inserting LNA nucleotides. After insertion of LNA nucleotides, melting temperatures are recalculated.
9. Modular Education for Interdisciplinary Systems Biology
10. Applied venomics of the cone snail species Conus consors for the accelerated, cheaper, safer and more ethical production of innovative biomedical drugs (CONCO)
Abstract: CONCO is an innovative post-genomic project dedicated to the discovery and development of novel biopharmaceuticals generated by the broad biodiversity of cone snails. The project aims at characterising from the genomic up to potential therapeutic properties all putative bioactive compounds that can be synthesised by a cone snail species.
The genome and transcriptome of Conus consors will be exhaustively studied. Large amounts of venom will be fractionated and submitted to proteomic studies to generate a biochemically characterised “”natural library”" of compounds. Large scale synthesis of each identified candidate will be achieved to form a “”synthetic library”" of compounds. The biological activity of these two libraries will be investigated on a panel of physiological targets that are recognised of therapeutic value. Selected hits will be optimised and validated in vivo. A publicly accessible web-based database will be developed and annotated to integrate and share all the knowledge generated by the project.
11. Array based sequencing-by-synthesis
12. Modeling of DNA-DNA interactions for PCR applications
13. Detection of conserved sequence motifs in eukaryotic genomes using comparative genomics approach
Program: ETF 2005
Abstract: The aim of the current project is to identify conserved functional elements from the mammalian genomes. Identification of evolutionarily conserved sequences helps to find a range of important functional elements that are otherwise difficult to detect, for example small RNA genes, promoters, transcription factor binding sites and other regulatory regions. For correct and efficient identification of conserved sequences we plan to test, compare and improve existing computational methods. The detection of conserved elements in studied genomes will be executed in three stages: alignment of syntenic genome regions, determination of evolutionarily conserved sequences and identification of functional sequence elements. The main emphasis is initially put into development of improved automatic methods for alignment of syntenic sequence regions, using orthologous genes as “anchors”. The conserved sequences identified within this project will be entered into dedicated database and visualized with help of the UCSC genome browser. All methods are initially tested in 3-4 well-studied genomic regions where experimental data is available as a set of positive controls. After verification the methods will be applied to the whole genome sequences.
14. SLIC-Biosensors in Molecular Diagnostics: Nanotechnology for the Analysis of species-specific Microbial Transcripts
Abstract: The development of the novel biosensor-based device for application in molecular diagnostics was based on combining two proprietary technologies, the SLIC-Nanobiosystem, the biosensor platform and RiboSEQ, a molecular target technology. The SLIC-Nanobiosystem consists of a self-assembled lipid bilayer membrane that integrates a synthetic ligand-gated ion channel (SLIC). The SLIC comprises a capture molecule that can specifically bind a given analyte, a process that is monitored via electrical impedance spectroscopy. It was shown recently that SLIC molecules can be designed to detect with high sensitivity antibody binding to antigens on a SLIC via modulation of the SLIC ion channel conductance [Angew. Chem. Int. Ed. 40, 1740-1743 (2001)]. With this system the effect from even a few channels can be resolved thus providing an ultra-sensitive, highly stable and versatile biosensor platform. The RiboSEQ platform based on the universal bacterial genomic target tmRNA encoded by the ssrA gene is a high copy number RNA target that has conserved and variable sequence signatures that can be exploited to develop nucleic acid tests with various levels of specificity for microbial identification. S. pneumoniae was selected as the model system for the development of the biosensor-based device. Additionally, it was envisaged that a novel sample preparation device that could be integrated with the biosensor to provide a homogenous assay format would be developed.
15. Haplotype structure of the human genome
Programm: SF 2004
Abstract: Current project is focused on analysis of haplotype structure of the human genome with the aim of improving methodology of large scale association studies. The study is performed with bioinformatic methods, using genotype data from public sources and also from collaborative projects with University of Tartu, The Sanger Institute, UK and GSF, Technical University of Munich. We initially focus on methods for modelling haplotype blocks and informative SNPs for their detection. The main task is to find a mathod for modelling haplotype blocks which would be a) effective, allowing description of haplotype variants with minimum number of tag SNPs b) universal, giving comparable results over different populations. We also study relationship between haplotype blocks and different genome features (location of genes, patterns of primary sequence). In the following years we plan to initiate additional projects for improving selection of controls for association studies and methods for statistical correction of the population stratification.
16. IT in higher education. Establishing the bioinformatics computer cluster
Financer: Eesti Infotehnoloogia Sihtasutus