Primer3 Tests for Windows Executables

Test for primer3.exe

Put these two files in the same directory as primer3.exe:
primer_mispriming_input
not_for_production.lib

At the command line run:

primer3.exe < primer_mispriming_input > primer_out.tmp

primer_out.tmp should be identical to primer_mispriming_output

Tests for ntdpal.exe

Run:

ntdpal.exe -g 3 -l 5 CGATATATTTGGTCTATA CGATATATTTCCGGTCTATA L

The output should be

|CGATATATTTGGTCTATA|  |CGATATATTTCCGGTCTATA| L score=10.00 len=16 |2,4|3,5|4,6|5,7|6,8|7,9|8,10|9,11|10,12|11,13|12,14|13,15|14,16|15,17|16,18|17,19|

Run:

ntdpal.exe -g 5 -l 3 GATATATTTGGTCTATA CGATATATTTCCGGTCTATA e

The output should be

|GATATATTTGGTCTATA|  |CGATATATTTCCGGTCTATA| e score=9.00 len=17 |0,3|1,4|2,5|3,6|4,7|5,8|6,9|7,10|8,11|9,12|10,13|11,14|12,15|13,16|14,17|15,18|16,19|