MultiPLX 2.1 is a tool for analyzing PCR primer compatibility and automatically finding optimal multiplexing (grouping) solution. It uses state-of-the-art nearest neighbour DNA binding thermodynamics to estimate possible unwanted pairings between PCR samples. This thermodynamic data is then used to distribute primers into groups that satisfy user defined set of constraints.
Stand-alone MultiPLX binaries, example file and README are available here.
Here is a correct primers file.
For further information please contact:
MultiPLX is a part of services provided by ELIXIR - European research infrastructure for biological information. For other services provided by ELIXIR's Estonian Node visit http://elixir.ut.ee/Main/Services.
How to cite MultiPLX: Kaplinski L, Andreson R, Puurand T, Remm M. MultiPLX: automatic grouping and evaluation of PCR primers. Bioinformatics. 2005 Apr 15;21(8):1701-2. [PubMed] [Full Text]
There have been 603887 submissions to this web site since January 2005.