qRTDesigner is a program for designing primers for real-time quantitative PCR assays.
qRTDesigner may take some time to work, so you may want to bookmark the results page or write down the results
ID and come back later. If you enter your email address, results ID will be sent to you when ready.
For further information please contact: .
There have been 3340 submissions to this web site since February 2008.
coordinates and ID-s
Number of primer pairs designed:
Amplicon melting temperature:
Primer melting temperature:
Maximal melting temperature difference between left and right primer:
Disallow primers with G nucleotide in their 5' end
Calculate primer secondary structures (mfold)
University of Tartu, Department of Bioinformatics 2018